Synodos for NF2 is a first-of-its-kind neurofibromatosis (NF) research collaboration dedicated to defeating the rare genetic disorder neurofibromatosis type 2 (NF2). Conceived and funded by the Children’s Tumor Foundation, the Synodos consortium brought together a multidisciplinary team of scientists from twelve world-class labs at academic and medical centers of excellence, who pledged to work closely together – sharing information, datasets, results and more – at every step in research development, with the goal of speeding up the drug discovery process. The goals of this effort are to find new approaches to the diagnosis and treatment of two primary NF2-related tumors (schwannoma and meningioma), and to publicly share data and tumor models to spur future drug discovery research in NF2.
On the occasion of NF2 Awareness Day – May 22, 2019 – all Synodos for NF2 data is now publicly available. This project has generated several types of data from NF2 tumor models of schwannoma and meningioma, including:
Low throughput drug screening data: A panel of rationally-selected small molecules and drugs such as mTOR and HDAC inhibitors were tested against human and mouse cell line models of meningioma and schwannoma.
Single-agent and combination high throughput drug screening data: A panel of 1912 drugs and drug-like molecules (the MIPE 4.0 library from NCATS) was screened in cell line models of meningioma and schwannoma as single drugs or in combinations.
Genomic variant data (structural rearrangement): Jumping library sequencing facilitates detection of chromosomal abnormalities such as pathogenic breakpoints. Both human and mouse cell line models of meningioma and schwannoma were profiled using this method.
Gene expression: RNAseq data were generated from an Nf2fl/fl mouse model dorsal root ganglion tissue and cell line models of meningioma and schwannoma. In addition, data were generated from cell line models of meningioma and schwannoma with a control, or one of 12 different single agents or combinations of small molecule inhibitors. Data from the 2017 release and the 2019 release are both now available for exploration.
Kinomics: Kinase profiling from NF2 mouse model dorsal root ganglion tissue several cell line models of meningioma and schwannoma after treatment with a control or one of seven small molecules. Data from the 2017 release and the 2019 release are both now available for exploration.
We encourage you to explore the Synodos NF2 project for more details, or the NF Data Portal to find these and other related data.
To learn more about the origins of the Synodos for NF2 project as well as other Synodos projects, visit: ctf.org/synodos.
